Effects of Vitrification Temperatures and Concentration of Cryoprotective Agents on the Cytoskeleton in Bovine Immature Oocytes
نویسندگان
چکیده
The objective of the research was to evaluate effect vitrification temperatures (VTs) and concentration cryoprotective agents (CPAs) on cytoskeleton bovine immature oocytes (BIOs). Bovine cumulus oocyte complexes (COCs) were collected divided randomly into five groups: (a) fresh control group, (b) vitrified in liquid helium (LHe; −269◦C with 5.6 M CPAs, (c) LHe (−269◦C 6.6 (d) nitrogen (LN; −196 ◦C (e) LN (−196◦C CPAs. results showed that developmental level BIOs group highest among four groups, maturation rate cleavage significantly higher than other three groups (P < 0.05). With further experiments, normal microfilament spindle compared groups. It concluded lower VT CPAs (5.6 M) could reduce damage cytoskeleton, which had a positive development after vitrification.
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ژورنال
عنوان ژورنال: Dokladi na B?lgarskata akademiâ na naukite
سال: 2023
ISSN: ['2367-5535', '1310-1331']
DOI: https://doi.org/10.7546/crabs.2023.04.18